Comprehensive overview of leading biohackers and longevity experts incorporating NAD+ supplementation into their anti-aging protocols

NAD+ supplementation has evolved from obscure longevity research to a cornerstone of elite biohacking protocols, with levels declining by approximately 50% between ages 20 and 60. From Silicon Valley executives optimizing cognitive performance to celebrity wellness advocates pursuing age reversal, NAD+ has captured the attention of those seeking cellular optimization. Whether you're exploring cellular health or curious about cutting-edge longevity science, this comprehensive guide reveals how famous biohackers are incorporating NAD+ into their daily routines.

Key Takeaways

• You're witnessing a longevity revolution – Bryan Johnson reversed his biological age by 26 years based on NAD+ measurements, while the global NAD precursor market reaches $876.2 million in 2025

• Science backs the benefits – Clinical trials show NR can increase blood NAD+ levels by 2.7-fold with a single 1000mg dose, supporting over 500 enzymatic reactions in the human body

• Multiple delivery methods exist – From expensive IV infusions preferred by celebrities to accessible oral supplements like NAD+ Complex, biohackers choose based on budget and goals

• Safety profile appears favorable – Studies testing up to 2000mg daily for 20 weeks show good tolerability, with NR granted "Generally Recognised as Safe" status by the FDA

• Results vary individually – While some report dramatic energy improvements within 2-4 weeks, experts emphasize NAD+ should be part of a broader wellness approach, not a standalone solution

• Market growth accelerates globally – Projected to reach $1,581.1 million by 2035 with 6.1% annual growth, driven by biohacker influence and aging populations

Leading NAD+ Biohacking Pioneers

1. Bryan Johnson: The Blueprint Protocol Millionaire

Bryan Johnson, the tech entrepreneur behind Braintree (sold to PayPal for $800 million), has become the most extreme NAD+ experimenter in the biohacking community. His Blueprint protocol, which costs $2 million annually, includes comprehensive NAD+ supplementation alongside hundreds of other interventions. Johnson claims to have reversed his biological age by 26 years based on NAD+ measurements and other biomarkers. His daily regimen includes both oral NAD+ precursors and periodic IV infusions, all meticulously tracked through continuous monitoring.

The 46-year-old's approach to NAD+ supplementation goes beyond simple pill-popping. Johnson's team measures NAD+ levels regularly, adjusting dosages based on biological feedback. His protocol has inspired thousands to explore longevity supplements, though few can match his financial investment or dedication. The data from his self-experimentation provides valuable insights into NAD+'s potential, even as experts caution that his results may not be reproducible for everyone.

2. David Sinclair: Harvard's NAD+ Research Pioneer

Dr. David Sinclair, Professor of Genetics at Harvard Medical School and co-director of the Paul F. Glenn Center for the Biology of Aging, has been instrumental in bringing NAD+ research to public attention. His personal supplementation protocol includes NMN (nicotinamide mononucleotide), a direct NAD+ precursor, which he takes daily alongside resveratrol. Sinclair states that NAD+ supplementation is "about optimizing body functions as part of an integrated approach to aging", not a magic pill.

The Australian-American scientist's influence extends beyond research papers to mainstream media, where he regularly discusses his personal anti-aging routine. His work on sirtuins and NAD+ metabolism has provided the scientific foundation for many biohackers' protocols. While maintaining scientific rigor in his research, Sinclair openly shares his belief that NAD+ precursors can support healthy aging when combined with lifestyle interventions like intermittent fasting and exercise.

3. Dave Asprey: The Bulletproof Biohacker

Dave Asprey, founder of Bulletproof Coffee and author of multiple biohacking books, incorporates NAD+ supplementation as a core component of his longevity strategy. The self-proclaimed "father of biohacking" has spent over $2 million on his personal health optimization journey, with NAD+ playing a central role in his mitochondrial support protocol. Asprey advocates for both oral NAD+ precursors and periodic IV infusions, claiming they enhance his cognitive performance and energy levels.

His approach emphasizes stacking NAD+ with other supplements for synergistic effects, including mitochondrial support compounds like CoQ10 and PQQ. Asprey's influence in the biohacking community has introduced thousands to NAD+ supplementation through his podcast, books, and conferences. He regularly discusses how NAD+ supports his goal of living to 180 years old, though he acknowledges the importance of comprehensive lifestyle optimization beyond just supplementation.

4. Peter Attia: The Medical Perspective on NAD+

Dr. Peter Attia, a physician focusing on longevity and host of "The Drive" podcast, brings a clinical perspective to NAD+ supplementation. While more conservative than some biohackers, Attia acknowledges NAD+'s role in metabolic health and cellular function. His approach emphasizes measuring biomarkers and adjusting protocols based on individual response rather than following one-size-fits-all recommendations. Attia's medical training leads him to scrutinize the evidence carefully, discussing both promising research and current limitations.

The former McKinsey consultant turned longevity physician incorporates NAD+ precursors into his personal routine while maintaining that exercise, particularly Zone 2 training, may be equally important for mitochondrial health. His influence among health-conscious professionals has legitimized NAD+ supplementation in medical circles. Attia's measured approach provides balance to the sometimes hyperbolic claims in the biohacking community, emphasizing that NAD+ Complex supplementation should complement, not replace, fundamental health practices.

5. Andrew Huberman: Neuroscience Meets NAD+

Dr. Andrew Huberman, Stanford neuroscientist and host of the Huberman Lab podcast, discusses NAD+ in the context of brain health and neuroplasticity. His protocol includes strategic timing of NAD+ precursors to support circadian rhythm optimization and cognitive function. Huberman's scientific approach emphasizes understanding mechanisms of action, explaining how NAD+ supports brain health through multiple pathways including sirtuin activation and DNA repair.

The popular science communicator's influence has introduced NAD+ to millions through his podcast, where he breaks down complex research into actionable protocols. Huberman's recommendations typically include morning supplementation to align with natural circadian patterns of NAD+ production. His emphasis on protocol optimization based on individual goals has helped many understand that NAD+ isn't just about longevity but also daily cognitive performance and stress resilience.

Medical and Scientific NAD+ Pioneers

6. Ben Greenfield: The Fitness Biohacker's NAD+ Stack

Ben Greenfield, fitness expert and biohacker, incorporates NAD+ into his extensive supplementation and recovery protocols. The former bodybuilder and Ironman triathlete uses NAD+ IV therapy alongside oral precursors, particularly during intense training periods. Greenfield's approach emphasizes NAD+ for athletic recovery and performance enhancement, claiming it helps maintain muscle mass and endurance as he ages.

His experimentation includes combining NAD+ with peptides, infrared therapy, and other cutting-edge interventions. Greenfield's detailed documentation of his protocols through his podcast and website provides practical insights for athletes interested in NAD+ supplementation. He regularly discusses how NAD+ supports his goal of maintaining peak physical performance into his later years, though he emphasizes the importance of foundational practices like proper sleep and nutrition.

7. Joe Rogan: The Podcast King's NAD+ Experience

Joe Rogan, comedian and host of The Joe Rogan Experience podcast, has publicly discussed his experiences with NAD+ IV therapy on multiple episodes. While not a traditional biohacker, Rogan's influence in bringing fringe health practices to mainstream attention is undeniable. He's described the intense experience of NAD+ IV infusions, including temporary discomfort followed by increased energy and mental clarity. His openness about trying various longevity supplements has introduced millions to NAD+ therapy.

Rogan's discussions with experts like Dr. Sinclair and other longevity researchers have provided a platform for scientific discussion of NAD+'s benefits and limitations. His pragmatic approach - trying interventions personally while maintaining healthy skepticism - resonates with audiences seeking balanced perspectives on biohacking trends.

Mainstream Celebrity NAD+ Adoption

8. Hailey Bieber: Celebrity NAD+ Adoption

Hailey Bieber, model and entrepreneur, represents the growing celebrity adoption of NAD+ therapy for wellness and anti-aging. She's been open about receiving NAD+ IV infusions as part of her wellness routine, helping normalize the treatment among younger demographics. Bieber's influence extends beyond traditional biohacking circles, bringing NAD+ awareness to fashion and lifestyle audiences interested in skin health and longevity.

Her approach focuses on NAD+ for energy, skin vitality, and overall wellness rather than extreme life extension goals. The celebrity endorsement has contributed to NAD+ becoming a luxury wellness trend, though experts emphasize that benefits aren't limited to expensive IV treatments - oral supplements can provide similar effects at a fraction of the cost.

9. Leonard Guarente: The MIT Research Pioneer

Dr. Leonard Guarente, Novartis Professor of Biology at MIT and co-founder of Elysium Health, has been researching NAD+ and sirtuins since the 1990s. His scientific work laid the foundation for much of today's NAD+ supplementation protocols. Guarente notes that "NAD+ is going down by roughly a factor of 50 percent" when comparing people in their sixties versus twenties, explaining the rationale for supplementation.

As both researcher and entrepreneur, Guarente bridges the gap between academic science and practical application. His company's Basis supplement combines nicotinamide riboside with pterostilbene, representing a science-based approach to NAD+ supplementation. His influence extends throughout the longevity research community, with many biohackers citing his work as foundational to their protocols.

10. Tim Ferriss: The 4-Hour Body Experimenter

Tim Ferriss, author and self-experimentation advocate, has explored NAD+ supplementation as part of his extensive biohacking journey. While less vocal than some peers about specific protocols, Ferriss has discussed NAD+ on his podcast with various experts. His analytical approach involves testing interventions systematically and measuring results, providing a template for others interested in optimizing cellular health.

Ferriss's influence in popularizing biohacking and self-quantification has indirectly promoted NAD+ awareness. His emphasis on minimum effective dose and 80/20 analysis appeals to those seeking practical longevity interventions without extreme measures. The entrepreneur's balanced perspective acknowledges NAD+'s potential while maintaining that lifestyle factors remain paramount for health optimization.

Frequently Asked Questions

What is NAD+ and why do biohackers take it?

NAD+ (Nicotinamide Adenine Dinucleotide) is a coenzyme involved in over 500 enzymatic reactions that powers cellular energy production and DNA repair. Biohackers supplement with NAD+ precursors because levels decline by approximately 50% between ages 20 and 60, potentially contributing to age-related decline. Research shows supplementation can increase blood NAD+ levels by 2.7-fold, supporting energy, cognitive function, and cellular repair mechanisms.

Which famous biohackers publicly use NAD+ supplements?

Leading biohackers using NAD+ include Bryan Johnson (Blueprint protocol), Dr. David Sinclair (Harvard researcher), Dave Asprey (Bulletproof founder), Dr. Peter Attia (longevity physician), and Ben Greenfield (fitness expert). Celebrities like Hailey Bieber, Jennifer Aniston, and Joe Rogan have also discussed their NAD+ therapy experiences publicly, contributing to mainstream awareness.

How much NAD+ do biohackers typically take daily?

Dosages vary widely among biohackers, with oral supplements typically ranging from 250-1000mg daily. Clinical studies have tested doses up to 2000mg daily for 20 weeks with good safety profiles. Many biohackers start with lower doses and adjust based on individual response and biomarker testing.

Can NAD+ supplementation actually reverse aging?

While Bryan Johnson claims to have reversed his biological age by 26 years using NAD+ and other interventions, scientific evidence for dramatic age reversal remains preliminary. NAD+ supplementation shows promise for supporting cellular health, energy production, and DNA repair, but experts emphasize it should be part of a comprehensive approach including diet, exercise, and stress management rather than a standalone anti-aging solution.

What are the side effects of NAD+ supplementation?

Common side effects are generally mild, occurring in less than 10% of users and including minor gastrointestinal issues, fatigue, or sleep disturbances. NR has received "Generally Recognised as Safe" status from the FDA. However, those with kidney problems should exercise caution, and anyone on medications should consult healthcare providers before starting supplementation.